![]() Accurate measurements of blood gases and acidbase status require an array of sophisticated laboratory equipment that is typically not available during field research such is the case for many studies on the stress physiology, ecology and conservation of elasmobranch fish species. However, it is not recommended for the assessment of gas tensions in shark blood. Results indicate that it is a useful tool for measuring blood pH and could be suitable for operation in most field settings. We validated the i-STAT system for the analysis of blood parameters in sandbar shark. Validation of the i-STAT system for the analysis of blood gases and acidbase status in juvenile sandbar shark (Carcharhinus plumbeus) KeywordsĬarbon dioxide tension, elasmobranch, oxygen tension, pH, portable clinical analyser Abstract Validation of the i-STAT system for the analysis of blood gases and acidbase status in juvenile sandbar shark (Carcharhinus plumbeus) Spring Valley Trout Farm (Langley, British Columbia, Canada) (49.0743, -122.559)ġ0.1093/conphys/cou037 This article contributed by:.In conclusion, the i-STAT system can easily generate fast results from rainbow trout whole blood, but many are inaccurate values. Application of conversion factors to correct i-STAT measurements is not recommended, due to significant effects of temperature, Hct and PCO2 on the measurement errors and complex interactions may exist. The algorithm that the i-STAT uses to calculate sO2 did not yield meaningful results on rainbow trout blood. Haematocrit was consistently underestimated by the i-STAT, while the measurements of Na+, PCO2, HCO3− and PO2 were variably inaccurate over the range of values typically found in fish. Our results indicate that pH was measured accurately with the i-STAT system over a physiological pH range and using the i-STAT temperature correction. This methodological comparison was performed at two temperatures (10 and 20☌), two haematocrits (low and high) and three PCO2 levels (0.5, 1.0 and 1.5%). The present study analysed a range of blood parameters in a model teleost fish (rainbow trout, Oncorhynchus mykiss) using the i-STAT system (CG8+ cartridges) and established laboratory techniques. Although originally conceived for clinical application and to replace robust but lengthy techniques, researchers have extended the use of the i-STAT system outside of humans and even to poikilothermic fish, with only limited validation. Portable clinical analysers, such as the i-STAT system, are increasingly being used for blood analysis in animal ecology and physiology because of their portability and easy operation. Results indicate that the i-STAT is not an appropriate tool for assessing most blood parameters in rainbow trout. This study validated the i-STAT system on rainbow trout blood under a broad range of conditions. The i-STAT system, a portable clinical analyzer, is increasingly being used to assess blood parameters in fish. Validation of the i-STAT system for the analysis of blood parameters in fish Keywordsīlood oxygen tension, blood pH, haematocrit, portable clinical analyser, rainbow trout Abstract
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